Comparison of throat and nasopharyngeal swab specimens for culture diagnosis of Bordetella pertussis infection.
نویسندگان
چکیده
During a 9-month period, we evaluated the relative sensitivity of throat and nasopharyngeal swab cultures for isolation of Bordetella pertussis. Of 38 pertussis cases, 36 (95%) had positive nasopharyngeal cultures, while only 16 of 36 (44%) had positive throat cultures. There were no cases of nasopharyngeal-negative, throat-positive cultures. The sensitivity of the direct fluorescent-antibody test was 70% when compared with culture.
منابع مشابه
Comparison of nasopharyngeal aspirates with swabs for culture of Bordetella pertussis.
Nasopharyngeal samples were collected from 117 children by aspiration from one nostril and by swab from the other one and cultured for Bordetella pertussis. Among 33 culture-positive specimens, there were 30 positive aspirates and 26 positive swab specimens. Aspirates are easily divided and saved for investigations with other assays which may further improve diagnostic sensitivity. As the aspir...
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BACKGROUND Advances in molecular laboratory techniques are changing the landscape of Bordetella pertussis illness diagnosis. Polymerase chain reaction (PCR) assays have greatly improved the sensitivity detection and the turnaround time to diagnosis compared to culture. Moreover, different respiratory specimens, such as flocked nasopharyngeal swabs (NPSs), nasopharyngeal aspirates (NPAs), and i...
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A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport syst...
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A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving the method a sensitivity of 100%. For B. pertussis, 58 of 520 patients (11.2%...
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BACKGROUND Accurate diagnosis of Bordetella pertussis infection is difficult. Polymerase chain reaction (PCR) tests are more sensitive than culture, but the reported sensitivity is variable. We prospectively compared the performance of culture, serology, and a multiplex PCR for the detection of B. pertussis. METHODS A total of 193 paired nasopharyngeal (NP) swab specimens were examined by bot...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 25 6 شماره
صفحات -
تاریخ انتشار 1987